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Figure 4 | International Archives of Medicine

Figure 4

From: Regulation of the epithelial sodium channel [ENaC] in kidneys of salt-sensitive Dahl rats: Insights on alternative splicing

Figure 4

Schematic representation of wildtype and the alternatively spliced forms α ENaC-a & -b. A. The genomic sequence of α ENaC wildtype, -a and -b forms. The alternatively spliced forms α ENaC-a & -b share the same splicing site [CCTGGG] which is located within exon VII. α ENaC-a & -b had 23 and 79 bases deleted respectively resulting in the formation of a premature stop codon. B. The protein sequence of α ENaC wildtype, -a and -b forms. The deletions of 23 and 79 bases respectively in α ENaC-a & -b introduced a premature stop codon and resulted in shorter proteins at the carboxy terminus by 199 in α ENaC-a and 216 amino acids in α ENaC-b, making α ENaC-a 499 amino acids [1497 bp] and α ENaC-b 482 amino acids [1446 bp] in length. The α ENaC-a and -b truncated proteins of approximately 55 and 53 kDa respectively, are identical to wildtype α ENaC up to amino acids 481 and 480 respectively, followed by 17 and 1 novel amino acids unique to the spliced form after which the stop codon terminates translation (adapted with permission from reference [33]).

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