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Figure 1 | International Archives of Medicine

Figure 1

From: Characterization of the epithelial sodium channel α subunit coding and non-coding transcripts and their corresponding mRNA expression levels in Dahl R versus S rat kidney cortex on normal and high salt diet

Figure 1

PCR analysis of α ENaC-a, -b, -c and -d variants versus α ENaC-wt in kidney cortex of Dahl S versus R rats on normal and high salt diet. Figure 1A) Specific sense and antisense primers were used for amplifying α ENaC-wt. α ENaC-wt fragment of 325 bp was amplified from kidney cortex of Dahl S and R rats fed normal and high salt diet. Figure 1B) Specific sense primers (missing the 23 bp unique to α ENaC-a) were utilized to amplify α ENaC-a. α ENaC-a fragment of 266 bp was amplified from kidney cortex of Dahl S and R rats fed normal and high salt diets. For a negative control, water without a cDNA template was used and for a positive control, α ENaC-wt primers were utilized to amplify α ENaC-wt fragment of 346 bp. Figure 1C) Specific antisense primers (missing the 79 bp unique to α ENaC-b) were utilized. α ENaC-b fragment of 278 bp was amplified from kidney cortex of Dahl S and R rats fed normal and high salt diet. Figure 1D) Specific primers for α ENaC-c were used to amplify a fragment of 59 bp. α ENaC-c was amplified from kidney cortex of Dahl S and R rats fed normal and high salt diet. Figure 1E) Specific primers for α ENaC-d were used to amplify a fragment of 99 bp. α ENaC-d was amplified from kidney cortex of Dahl S and R rats fed normal and high salt diet. C (Control): water without cDNA template; wt: wildtype α ENaC primers; H: High salt diet; N: Normal salt diet. The sizes of the expected PCR products are indicated.

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