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Figure 3 | International Archives of Medicine

Figure 3

From: Characterization of the epithelial sodium channel α subunit coding and non-coding transcripts and their corresponding mRNA expression levels in Dahl R versus S rat kidney cortex on normal and high salt diet

Figure 3

Quantitative RT-PCR analysis of RNA expression of α ENaC-wt and the alternatively spliced forms -a, -b, -c and -d in kidney cortex of Dahl S and R rats fed normal and high salt diet. Figure 3A) Specific primers for α ENaC-wt were used to amplify cDNAs from kidney cortex of Dahl S and R rats on normal and high salt diet. A significant increase (P < 0.05) in α ENaC-wt expression was observed in Dahl R versus S rats. No significant dietary impact on the expression levels of α ENaC-wt was noticed in Dahl S or R rats. Figure 3B) Specific primers unique to α ENaC-a were used to amplify cDNAs from kidney cortex of Dahl S and R rats on normal and high salt diet. A significant increase (P < 0.05) in α ENaC-a expression was observed in Dahl R versus S rats. No significant dietary impact on the expression levels of α ENaC-a was noticed in Dahl S or R rats. Figure 3C) Specific primers unique to α ENaC-b were used to amplify cDNAs from kidney cortex of Dahl S and R rats on normal and high salt diet. A significant increase (P < 0.05) in α ENaC-b expression was observed in Dahl R versus S rats. Additionally, a significant increase in the expression levels of α ENaC-b was observed on high versus normal salt diet in Dahl R, but not S rats. Figure 3D) Specific primers unique to α ENaC-c were used to amplify cDNAs from kidney cortex of Dahl S and R rats on normal and high salt diet. Only a slight, insignificant increase in α ENaC-c expression was observed in Dahl R versus S rats. No significant dietary impact on the expression levels of α ENaC-c was noticed in Dahl S or R rats. Figure 3E) Specific primers unique to α ENaC-d were used to amplify cDNAs from kidney cortex of Dahl S and R rats on normal and high salt diet. Only a slight, insignificant increase in α ENaC-a expression was observed in Dahl R versus S rats. α ENaC-a, -c and -d are lower abundant transcripts. Only α ENaC-b exceeded α ENaC-wt expression by ~32 fold. Bars represent mean +/- SEM, *: denotes P < 0.05 between Dahl R and Dahl S, #: denotes P < 0.05 in transcript abundance on normal and 4 week high salt diet in Dahl R, Dahl R: Dahl salt-resistant, Dahl S: Dahl salt-sensitive. All α ENaC transcript levels were normalized against the house keeping gene PgK. N = 6 rats/group and the results are the average of 3 independent experiments.

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