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Table 1 Primers used for reverse transcription polymerase chain reaction and expected sizes of cDNA fragments.

From: Characterization of the epithelial sodium channel α subunit coding and non-coding transcripts and their corresponding mRNA expression levels in Dahl R versus S rat kidney cortex on normal and high salt diet

Accession ID Amplicon Size Primers used
α ENaC-wt 325 bp Sense: 5'-TCATGCTGCTACGCCGGTTCC-3'
   Antisense:5'-TCCATCAGTTTACAAGGGAG-3'
α ENaC a 266 bp Sense: 5'-CAGAGCTCCTGGGGGCGCCTT-3'
   Antisense: 5'-TCCTTCTGTCACGATGGTCA-3'
α ENaC-b 278 bp Sense: 5'-GGATGATGGTGGCTTCAACT-3'
   Antisense: 5'-ACACCCAGGAGCTCTGCTTT-3'
α ENaC-c 59 bp Sense: 5'-GCTCCTGGGTGTGATCAACTA-3'
   Antisense: 5'-AAAGAAGGTGGTGGGGATGT-3'
  59 bp Sense: 5'-GGATGATGGTGGCTTCAACT-3'
   Antisense: 5'-AAAGAAGGTGGTGGGGATGT-3'
α ENaC-d 99 bp Sense: 5'-CCACGCGTCCGAAGAAAC-3'
   Antisense:5'-AAAGAAGGTGGTGGGGATGT-3'
  99 bp Sense: 5'-CCACGCGTCCGAAGAAACTG-3'
   Antisense:5'-TAAAGAAGGTGGTGGGGATG-5'
CK479583 767 bp Sense: 5'-GCCCCAGCAGGGCATGACCC-3'
   Antisense: 5'-CTAGAGTAGCATAGGCAGGT-3'
CK475461 814 bp Sense: 5'-TCCAAGTATACACAGCAGGT-3'
   Antisense: 5'-TACAGGAGTCAGGCTGTGAG-3'
CK364785 764 bp Sense: 5'-GGGGCTATTGCTATTATAAA-3'
   Antisense:5'-CCACACAGAGGGCTCGGCGGG-3'
CK475819 748 bp Sense: 5'-GATTCCCGGGATCTTGGA-3'
   Antisense: 5'-AGGCTGACCATCGTGACAG-3'
CB690980 376 bp Sense: 5'-CCACGCGTCCGAAGAAAC-3'
   Antisense: 5'-AAAGAAGGTGGTGGGGATGT-3'
Pgk 263 bp Sense: 5'-GCTGCAGAACTCAAATCTCT-3'
   Antisense:5'-TGTGTGCAGTCCCAAAAGCA-3'