Table 2 Most common chemicals used in the creation of cell lines
maintenance and growth media | Dulbecco's modified Eagles medium (DMEM)-with 2 mM L-glutamine, 100 units/ml |
|---|---|
| Â | Penicillin and 100 ug/ml streptomycin, 2.5 ug/ml |
| Â | Amphotericin B (An antimicrobial cocktail to reduce chance of infection) |
| Â | 20% of Fetal Calf Serum- 10% v/v-decomplemented at 56'C for 45 min, (A growth medium containing several important growth factors) |
| Â | Phosphate buffer saline (for isotonic washes and mechanical reduction in microbial load) |
| Â | 7% Dimethylsulfoxide (DMSO for cryo-preservation) |
| Â | 93% Fetal Calf Serum |
separation of cells explants for cell lines | Trypsin 0.05%, 0.01% bovine pancreatic trypsin (to allow detachment of cells from underlying substrate- plastic or collagen) |
| Â | Na2EDTA |
| Â | Defined keratinocyte serum free medium |