Skip to main content

Table 2 Most common chemicals used in the creation of cell lines

From: Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): Techniques of tissue harvesting and propagation

maintenance and growth media Dulbecco's modified Eagles medium (DMEM)-with 2 mM L-glutamine, 100 units/ml
  Penicillin and 100 ug/ml streptomycin, 2.5 ug/ml
  Amphotericin B (An antimicrobial cocktail to reduce chance of infection)
  20% of Fetal Calf Serum- 10% v/v-decomplemented at 56'C for 45 min, (A growth medium containing several important growth factors)
  Phosphate buffer saline (for isotonic washes and mechanical reduction in microbial load)
  7% Dimethylsulfoxide (DMSO for cryo-preservation)
  93% Fetal Calf Serum
separation of cells explants for cell lines Trypsin 0.05%, 0.01% bovine pancreatic trypsin (to allow detachment of cells from underlying substrate- plastic or collagen)
  Defined keratinocyte serum free medium