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Figure 1 | International Archives of Medicine

Figure 1

From: Midkine as a factor to counteract the deposition of amyloid β-peptide plaques: in vitro analysis and examination in knockout mice

Figure 1

Midkine binding to Aβ. Specific interactions of midkine with Aβ were examined using the Biacore system. (A), Sensorgrams for the binding of human midkine (hMK) to Aβ (left) and the calibration curve (right). Various concentrations of hMK (0.62 μg-20 μg/ml) were injected onto an Aβ- immobilized sensor chip. The arrows indicate the beginning of the association phase initiated by the injection of hMK and the beginning of the dissociation phase initiated with the running buffer. RU, response units (B), Binding of hMK, hPTN (left) and mouse midkine (mMK) (middle) to Aβ, and inhibition of the binding of midkine to Aβ by heparin (right). hMK, hMKC-half (MK-C), hMK N-half(MK-N), hPTN, hPTN C-half (PTN-C), hPTN N-half (PTN-N), mMK, mMK mutants (m1: R78Q; m2: K83Q/K84Q; m3: R78Q/K83Q/K84Q) were injected onto an Aβ-immobilized sensor chip in concentration of 5 μg/ml. In the inhibition assay with heparin, hMK (5 μg/ml) and heparin (0-25 μg/ml) in the running buffer were injected into the flow cell.

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